Catalog Number. Log Out. Show More. QIAprep 2. For high-purity plasmid minipreps. Log in to see your account pricing. This product is not intended for the diagnosis, prevention, or treatment of a disease. Spin column handling options — A. Spin column handling options — B. QIAprep membrane technology eliminates time consuming phenol — chloroform extraction and alcohol precipitation, as well as the problems and inconvenience associated with loose resins and slurries.
High-purity plasmid DNA eluted from QIAprep spin columns is immediately ready to use — there is no need to precipitate, concentrate, or desalt. The QIAprep procedure. An optional pH indicator allows easy determination of the optimal pH for DNA binding to the spin column.
The procedure can be fully automated on the QIAcube Connect. For optimal results it is recommended to use this product together with QIAvac 24 Plus. Each step of the protocol execution is recorded to accelerate reporting by generating a comprehensive run report.
Download more information. QIAquick Kits contain a silica membrane assembly for binding of DNA in high-salt buffer and elution with low-salt buffer or water. The purification procedure removes primers, nucleotides, enzymes, mineral oil, salts, agarose, ethidium bromide, and other impurities from DNA samples see figure " Complete primer removal after PCR. M : markers. Silica-membrane technology eliminates the problems and inconvenience associated with loose resins and slurries.
Specialized binding buffers are optimized for specific applications and promote selective adsorption of DNA molecules within particular size ranges.
To enable faster and more convenient sample processing and analysis, gel loading dye is provided. GelPilot Loading Dye contains three tracking dyes xylene cyanol, bromophenol blue, and orange G to facilitate the optimization of agarose gel run time and prevent smaller DNA fragments migrating too far see figure " GelPilot Loading Dye.
The QIAquick and MinElute systems use a simple bind-wash-elute procedure with spin columns or a vacuum manifold. Binding buffer is added directly to the PCR sample or other enzymatic reaction, and the mixture is applied to the QIAquick spin column.
An incorrect binding-mixture pH can occur if the agarose gel electrophoresis buffer was frequently used or incorrectly prepared. Nucleic acids adsorb to the silica membrane in the high-salt conditions provided by the buffer. Impurities are washed away and pure DNA is eluted with a small volume of low-salt buffer provided or water, ready to use in all subsequent applications.
QIAquick spin columns are designed to provide two convenient handling options. QIAvac Manifold with luer connectors. QIAvac 24 plus.
DNA fragments purified with the QIAquick system are ready for direct use in all applications, including sequencing, microarray analysis, ligation and transformation, restriction digestion, labeling, microinjection, PCR, and in vitro transcription.
It is difficult to predict whether a DNA fragment larger than 10 kb can be efficiently recovered, because this depends on base composition as well as fragment size. However, please note that it will become less likely to recover your sample the larger the fragment size is.
As we cannot guarantee recovery of fragments larger than the maximum cutoff size, we do not recommend to purify such fragments using QIAquick Kits. Buffer PE did not contain ethanol. Ethanol must be added to Buffer PE concentrate before use. Repeat procedure with correctly prepared Buffer PE. DNA will only be eluted efficiently in the presence of low-salt buffer e. A variety of modified silica gel surfaces and optimized binding buffers are used to obtain maximum discrimination between nucleic acids during adsorption and washing steps.
Silica gel membranes are particularly well-suited for use in spin columns or multiwell units designed for high-throughput procedures. Nucleic acids are adsorbed to the silica gel membrane in the presence of chaotropic salts, which remove water from hydrated molecules in solution.
Polysaccharides and proteins do not adsorb and are removed. After a wash step, pure nucleic acids are eluted under low- or no-salt conditions in small volumes, ready for immediate use without further concentration. QIAGEN silica gel membrane technology yields high-purity nucleic acids suitable for most molecular biology and clinical research applications, such as restriction digestion, ligation, labeling, amplification, and radioactive and fluorescent sequencing.
Purification of nucleic acids with silica gel membrane products is fast, convenient, and economical. QIAGEN silica gel membrane technology also avoids the handling inconveniences of loose silica resins or slurries and the problem of silica carryover which can interfere with downstream applications.
The use of magnetic particles allows a rapid purification procedure to be performed, from the initial binding of target molecules e. Nucleic acids are isolated from lysates through binding to the magnetic particles in the presence of a chaotropic salt, which removes water from hydrated molecules in solution.
The particles are separated from the lysates using a magnet. The nucleic acids are then efficiently washed and eluted under low- or no-salt conditions in small volumes of elution buffer.
Magnetic particle technology combines the speed and efficiency of silica-based DNA purification with convenient handling and ease of automation. Magnetic particle technology removes the need for centrifugation or vacuum processing, eliminating tedious and time-consuming processing steps.
Knowledge Hub. Plasmid Resource Center. QIAGEN anion-exchange, Plasmid Plus , silica gel membrane, and magnetic particle technologies QIAGEN technologies have revolutionized nucleic acid purification by substantially reducing preparation times and eliminating the need for costly equipment, such as ultracentrifuges, and toxic chemicals, such as phenol. Purified nucleic acids are of the highest possible quality and are highly suitable for sensitive downstream biological applications, such as transfection, microinjection, sequencing, and gene therapy research.
This property optimizes the separation of nucleic acids — highly negatively charged, linear polyanions — from other substances and provides the highest possible nucleic acid quality. It also allows the separation of different classes of nucleic acids from one another by step elution using simple, pH and salt optimized buffers.
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